Supplementary MaterialsFigure 2source data 1: Supply data of the relative distributions of significant neural responses in odor-guided go/no-go task. of the correlation coefficients and cell-shuffled data, determined for different pairs of cells. Neuronal response profiles were more related between odor cues for combined responses from your same neuron (reddish) than for reactions of Neohesperidin two different neurons (black) (p 10?13, two-sample KolmogorovCSmirnov test). We recorded the spiking activity of 270 vTT cells from six mice (Furniture 1 and ?and2;2; recording positions are demonstrated in Number 1B) while they performed the proceed/no-go task. As the vTT receives direct inputs from mitral and tufted cells of the olfactory bulb, we first focused on whether vTT cells exhibited odor cue-responsive activity during odor presentation. We observed that a subset of vTT cells improved their firing prices during the smell presentation stage during both move and no-go studies (a good example is normally shown in Amount 1C). To quantify the dependence of firing price on the smell presentation stage, we computed firing rate adjustments from baseline (pre-odor cue period, 1.2 to at least one 1 s prior to the smell port entrance) in sliding bins (width, 100 ms; stage, 20 ms) utilizing a recipient operating quality (ROC) analysis approach. We computed the area beneath the ROC curve (auROC) at every time bin (spike data had been aligned towards the onset of smell valve starting). auROC beliefs ranged from ?1 to +1, with positive and negative beliefs reflecting elevated and reduced firing prices in accordance with baseline, respectively. We further driven auROC value significance using a permutation test (see Materials and methods). Table 1. Fundamental info in the odor-guided proceed/no-go task. test). Changes in firing rate in individual vTT cells exhibited related time courses for proceed and no-go tests. We quantified this by calculating the correlation coefficients of response profiles between correct proceed trials and right no-go trials for each cell (top lines in Number 1E). This analysis revealed that the activity of vTT cells was strongly correlated between proceed and no-go odor cue presentation phases, whereas different cell pairs did not exhibit this correlation (bottom lines in Number 1E, p 10?13, two-sample KolmogorovCSmirnov test). These results suggest that individual vTT cells did not represent odor cue variations between proceed and no-go tests during odor presentation phases. We consequently hypothesized that firing activity primarily reflected animal behavior and was dependent on task context. Behavior-specific activity of vTT cells in the odor-guided proceed/no-go task Many vTT cells exhibited an increase in firing rate during specific behaviors over the course of the odor-guided proceed/no-go task (Number 2figure product 1A). Time intervals between behavioral events (the time from odor valve opening until the Neohesperidin mouse withdrew its snout from your odor port, and the time from odor port withdrawal until reward slot access) also assorted across tests (coloured shaded areas in Number 2figure product 1A). To develop an overall firing profile accounting for this variability, we created event-aligned spike histograms (EASHs) (Ito and Doya, 2015). An EASH was derived by linearly scaling time intervals between behavioral events in each trial and the median interval for all trials (Figure 2figure supplement 1B, see Materials and methods). The EASHs clearly demonstrated that individual vTT cells were activated during different behavioral epochs (between-event intervals), such as when mice were poking the odor port in the approach epoch (plots in bottom left, Figure 2A) and during the odor-sampling epoch Neohesperidin (plots second from the bottom left, Figure 2A). Open in a separate window Figure 2. Tuning of vTT cells to distinct behaviors in the odor-guided go/no-go task.(A) Left panel: examples of event-aligned spike data for five representative cells tuned to specific behaviors. Event-aligned spike histograms were calculated using a 20 ms bin width and smoothed by convolving spike trains with a 60 ms wide Gaussian filter. Gray shading indicates the approach epoch (500 ms before odor port entry), yellow shading indicates the odor-sampling epoch (from entry into the odor port to exiting the odor port), orange shading shows the shifting epoch (from exiting the smell port to admittance into the drinking water slot), light blue shading shows the waiting around epoch (drinking water reward hold off, 300 ms before drinking water valve was fired up), blue shading shows the consuming epoch (1000 ms following the drinking water valve was fired up). Right -panel: auROC ideals had been determined from event-aligned spike data (aligned by smell valve starting) for many cells, sorted from the peak period for auROC ideals. Each row corresponds to PRKD3 1 cell. auROC ideals had been calculated by evaluating proceed correct tests to baseline (pre-odor cue period, 1.2 to at Neohesperidin least one 1 s before smell port admittance) in sliding bins (width, 100 ms; stage, 20 ms). Vertical white lines reveal transitions between behavioral epochs, including smell port admittance (related to smell valve starting), smell port exit, drinking water port admittance, and drinking water valve opening. The colour.